DNA FROM TISSUE AND FORMALIN-FIXED, PARAFFIN-EMBEDDED TISSUE (FFPE) 

Extraction of total DNA from
formalin-fixed paraffin-embedded (FFPE), tissue and forensic samples
with the MagCore® System

Tissue_MagCore_FFPE

Total DNA purified by the MagCore Tissue and FFPE Tissue Kits is ideal for sensitive downstream applications such as PCR, Real-Time PCR, and multiple PCR. Users can count on its reliability to routinely operate total DNA extraction.

Two protocols have been devised and optimized for different sizes of tissues: 2 hrs for small samples/ 16 hrs for large samples.

Archived Formalin-Fixed, Paraffin-Embedded (FFPE) tissues are valuable samples to profile gene expression or study diseases.  Amplifying DNA from scarce and low-quality FFPE tissue can be tricky.
The MagCore Genomic DNA FFPE process is designed for purification of total DNA from FFPE tissues with the MagCore instruments. It features One-Step Heating, to melt the paraffin and lyse tissue samples at the same time without applying harmful reagents such as xylene.

FFPE_samples_magcore

The surface area of the FFPE tissue sample measured as shown in examples. Sample amount of preparation can be 1-5 scrolls, each with a thickness up to 5μm. One FFPE scroll could be enough to analyze if the surface area is over 200 mm2.

GENOMIC DNA FROM TISSUE

  • Suitable for various sample types, especially of archival and forensic origin
  • High-quality DNA is ready to be used for downstream applications

GENOMIC DNA FROM FFPE TISSUE IN ONE STEP

  • De-paraffinization without xylene
  • Effective mixing of FFPE samples during processing
  • Complete within 3 hours

GENOMIC DNA FROM TISSUE SAMPLES

  • Animal tissue
  • Stool & Swab
  • Feed-soil samples
  • Forensic samples

VIDEO:

Here we show the complete workflow of DNA extraction and purification from FFPE samples - starting with neutralizing paraffin and ending with the DNA purification.

The MagCore® Genomic DNA FFPE One-Step Kit is used with the  MagCore Nucleic Acid Extractor.

  • Deparaffinization without xylene
  • Thermostable cap assures optimal incubation and processing in the reaction well
  • Extensive mixing of  the lysis buffer and FFPE sample
  • Process duration: approx. 3 hours

CONSISTENT DNA EXTRACTION PERFORMANCE

Superior yield as compared to a competitor's product

2h_heating_magcore

Extraction from small samples yields more DNA with 2 h heating protocol as compared to a competitor's product.
Genomic DNA Tissue Kit - 401 vs. competitor (Q).

Extracted DNA suitable for PCR and real-time PCR

DNA_suitable_for_PCR_MagCore_vs_Qiagen

PCR products of DNA purified by the MagCore System compared to a competitor's platform are confirmed by 1.5% agarose gel electrophoresis.

MagCore Genomic DNA Tissue Kit -401 performs better than its competitor with the 2h and the 16 heating protocol.

2h heating protocol - ideal for small samples,

16h heating protocol for large samples

16h_heating_MagCore

Extraction from small samples yields more DNA with 16 h heating protocol as compared to a competitor's product. Genomic DNA Tissue Kit - 401 vs. competitor (Q).

Tissue Sample Sizes

2h_16h_heating_protocol_MagCore

Different sizes of tissue samples were processed by either the 2-hour or 16-hour heating program.

Purified DNA - ideal for real-time PCR processing

PCR_comparison_MagCore_2h_16h

MagCore-treated samples have mostly lower (better) CT values as compared to samples processed with a competitor's product, regardless if the 2-hour or 16-hour heating protocol is applied.

DNA yield from a variety of mouse tissues

Organs_DNA_MagCore

DNA yield gained from 10 mg of mice tissue samples and 0.25 cm tail

  • Paraffin-Embedded Tissue
  • Needle-like FFPE Tissue Slices
  • Glass-slide Samples
  • Swab Sample
  • Solid Animal Tissue
  • Stool Sample
  • Feed-soil Sample
  • Dried Blood Spot
  • Cigarette Butts
  • Hair Roots
  • Chewing Gum
  • Betel Nut Residue
  • Saliva
  • Cultured Yeast

Suitable Tissue and Forensic Sample Amount: up to 40 mg
Processing Time: 33 min for 400μl samples
Contents: pre-filled cartridges, filter column sets, GT buffer, proteinase K, PK storage buffer, disposable tip & holder sets, sample tubes, elution tubes
Shelf life: 12 months

Suitable Samples Type: FFPE (Formalin-fixed, Paraffin-embedded) tissue samples
Processing time:  175 min for samples with 2-hour heating
16 hrs and 52 min for samples with 16-hour heating
Contents: thermostable cap, SULA oil, pre-filled cartridges, filter column sets, GT buffer, proteinase K, PK storage buffer, disposable tip & holder sets, sample tubes, elution tubes
Shelf life: 12 months

Cartridge Code: 401

MGT-01 (36 tests / kit )
MGT-02 (96 tests / kit )

Cartridge Code : 405

MGF-01 (36 tests / kit )
MGF-03 (72 tests / kit )